Yes you can with Dolphin an emulator
It is in its early stages and does not work with everything ive tested it with about 6 games and only 2 work but they do work. Not properly set out the controllers yet but you can use your keyboard and mouse and other controllers and of course you can use the Wiimote (but I am yet to even try that )
http://www.dolphin-emu.com/news.php